Fig. 2.
The KD values of the histamine H1 receptor antagonists by ACE2-HEK293T/CMC model. (A) ACE2-HEK293T/CMC breakthrough curves of doxepin and the regression curves achieved by plotting mlapp versus 1/[A]. (B) The concentrations of drugs were 1 × 10-7, 2 × 10-7, 4 × 10-7, 8 × 10-7, 1 × 10-6, 2 × 10-6 M (from bottom to top), respectively. (C) The chromatograms of histamine H1 receptor antagonists on the ACE2-HEK293T/CMC column and the KD values of the drugs calculated by relative standard method. Experiments were repeated three times.