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. 2020 Sep 15;38(2):437–448. doi: 10.1093/molbev/msaa232

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — trn expression expands in the clasper primordia upon h RNAi knockdown in D. melanogaster. (A) A schematic of genital development based on DAPI at stage 5 (Hagen et al. 2019). (B) h mRNA in situ hybridization in D. melanogaster w¹¹¹⁸. (C) trn mRNA in situ hybridization on UAS parental control and (D) trn mRNA in situ hybridization upon h RNAi knockdown at stage 5. Both trn in situ hybridizations were carried out in parallel and allowed to develop for the same length of time. h RNAi results in a distortion of trn expression at the base of the claspers compared with the UAS control (black arrows). CL, claspers; A, aedeagus. N = 5 for each experiment.