Table 1.
Summary of Candidate Regions Underlying Clasper and Posterior Lobe Divergence.
| Candidate Region | Phenotypic Effect Sizea (%) | Number of Expressed Genes |
||||||
|---|---|---|---|---|---|---|---|---|
| Total Number of Genesb | Total | Diffc | Upregulatedc |
Genes Tested by RNAi in D. mel |
||||
| Dsim w 501 | Dmau D1 | Total | Developmental Candidatesd | |||||
| C0 | 11 (20) | 99 | 69 | 14 | 5 | 9 | 7 | sgl |
| C1 | 6 (21) | 58 | 35 | 6 | 4 | 2 | 32 | hairy, Cpr66D, Gug, Mcm7, foi |
| P1 | 4 | 99 | 69 | 14 | 5 | 9 | 7 | — |
| P2 | 6 | 7 | 2 | 1 | 1 | 0 | 7 | — |
| P3 | 6 | 71 | 49 | 10 | 5 | 5 | 5 | — |
| P4 | 5 | 52 | 38 | 5 | 3 | 2 | 2 | — |
| P5 | 9 | 93 | 67 | 13 | 7 | 6 | 0 | — |
a
The phenotypic effect size is calculated as a percentage of the difference in phenotype between the parental strains. Brackets in C regions indicate effect size for clasper bristle number.
b
Protein-coding orthologs in Drosophila melanogaster R6.24.
c
Differentially expressed between Dmau D1 and Dsim w501, Padj (FDR) < 0.05.
d
Genes that significantly affect either clasper size, bristle number, or posterior lobe size compared with both UAS and driver controls (P < 0.05) after RNAi knock-down are considered developmental candidates.