Figure 2.
The workflow of sgRNA synthesis. The multiple oligos with a promoter sequence (red) and an overlap sequence (green) on either side of the target sequence are hybridized with a single complementary oligo that shares the overlap sequence.
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.