Table 2.
The off-target labeling of RR3131
| Strains | Locations | Labeling | Target sequence |
|---|---|---|---|
| RR722 | 21722 | GCTTTTTAGGATATCGTCCCNGG | |
| RR3131 | 21698 | off target | GCTTTTTAAGATATCGTCCCAGG |
| RR722 | 59529 | GCGGTATCCACCCCCACTGCNGG | |
| RR3131 | 60913 | off target | GCAGTATCCACCCCCACTGCAGG |
| RR722 | 86065 | GTTACATTACACACAAACTTNGG | |
| RR3131 | 86656 | off target | GTTACATTACACACAAATTTTGG |
| RR722 | 94393 | GGGGCGTAAATTCTTAACATNGG | |
| RR3131 | 151264 | off target | GGAGCGTAAATTCTTAACATTGG |
| RR722 | 253327 | CGAAGGGATAAATATTGCGANGG | |
| RR3131 | 316470 | off target | TGAAGGGATAAATATTGCGATGG |
| RR722 | 270963 | TAGCACTTAAAAGAGGAATGNGG | |
| RR3131 | 334078 | off target | TGGCACTTAAAAGAGGAATGGGG |
| RR722 | 219206 | TTGTTTTACGATATAATACGNGG | |
| RR3131 | 281336 | no label | TTGTTTTGCGATATAATACGAGG |
| RR722 | 296956 | TAATCAAGCATTAGATAGCTNGG | |
| RR3131 | 359914 | no label | GCGTAAAGCATTAGATAGCTTGG |
Two rows are shown for each of eight probes that did not have a perfect hit in the RR3131 genome. The second row is the designed probe named for its hit location on the RR722 genome. The upper row is the sequence found in the RR3131 strain, and named for its location. Bold indicates a PAM sequence motif (NGG). Red indicates a base that does not match the designed probe. The last two probes did not have a label seen consistently in the aligned data.