Figure 1.

Evaluating translation inhibitors in vivo. (A) Single injection done to retroorbital cavity around the eye. (B) Both translation inhibitors show similar efficiencies and uptake times following injection into the bloodstream. Using sucrose gradient profiles of the mouse liver as an example, we demonstrate that harringtonine depletes polysomes while co-injection with cycloheximide preserves polysomes. (C) Harringtonine performance in 7 organs. It effectively stalls ribosomes at the vicinity of start codons. Post-injection incubation time was 5 min except testes, where up to 30 min were required to pass the blood-tissue barriers. Zero corresponds to the start of the open reading frame. 100 nucleotides from the 5′-UTR were added to allow mapping footprints over the start codon.