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. 2021 Jan 6;49(2):1133–1151. doi: 10.1093/nar/gkaa1260

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — siRNA in HeLa cells differentially affects the alternative splicing isoforms of its target gene. (A) Gene models of SRSF4 (SRp75). Intron 2 is retained in IR isoform. REF, reference transcript. siRNA binding site is shown by a pentagon (siSRSF4). (B) RT-PCR showing SRSF4 isoform relative abundance changes induced by the action of siSRSF4. (C, D) Total levels of SRSF4 are downregulated by siSRSF4 as revealed by qPCR (C), REF is also downregulated (D) but IR isoform remains unchanged (E). Primers are shown by arrows. Dashed arrows represent primers spanning exon junctions. Primers are listed in Supplementary Table S2. Data represent means ± standard deviation (n = 3). Student's t-test: ***P < 0.001; **P < 0.01; ns, not significant.