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. 2020 Dec 24;49(2):1094–1113. doi: 10.1093/nar/gkaa1226

Figure 2.

Figure 2.

Identification of early assembly intermediates with PRPF31 mutant proteins. (A–C) Proteomic analyses of the partners of PRPF31-WT (A), GFP-PRPF31-A216P (B) and PRPF31-KA/AR (C). First and second experiments were done with three conditions (L1/L2: light label for control done with parental HeLa cells; M1/M2: medium label for GFP-PRPF31-WT; H1: heavy label for GFP-PRPF31-A216P in the first experiment; H2: heavy label for GFP-PRPF31-KA/AR in the second experiment). Full hit list with Significance B values are given in Supplementary Table S2. Legends as in Figure 1 for the graphic representation. SILAC ratio shown in A is mean of the two experiments. (D) Bar plot comparing SILAC ratios shown in A, B and C for a few selected partners. In each experiment, the normalization is done by dividing the SILAC ratio of the protein of interest with that of PRPF31. Blue bars are for PRPF31-A216P, red bars for PRPF31-KA/AR and green bars for PRPF31-WT.