. 2021 Jan 11;118(3):e2018027118. doi: 10.1073/pnas.2018027118

Table 1.

D5 IgG neutralizes tier-1 and tier-2 viruses across clades in TZM-bl/FcγRI cells

Virus	Tier	Clade	ID₅₀ in TZM-bl cells, µg/mL	ID₅₀ in TZM-bl/FcγRI cells, µg/mL
SVA-MLV	Negative control		>25	>25
X2278	Tier 1B	Clade B	>25	0.53
246-F3	Tier 2	Clade AC	>25	>25
CNE55	Tier 2	CRF01_AE	>25	0.88
TRO.11	Tier 2	Clade B	>25	4.8
BJOX2000	Tier 2	CRF07_BC	>25	0.53
CH119	Tier 2	CRF07_BC	>25	1.8
Ce1176	Tier 2	Clade C	>25	7.0
25710	Tier 2	Clade C	>25	0.36
Ce0217	Tier 2	Clade C	>25	0.43
X1632	Tier 2	Clade G	>25	0.71

Viruses were pseudotyped with Env from various HIV-1 strains. ID₅₀ was determined using a validated neutralization assay (63–66). SVA-MLV is lentivirus pseudotyped with murine leukemia virus (MLV) envelope to detect nonspecific inhibition.