Fig. 7.

DT treatment of Zbtb46^GFP CLEC4C-DTR⁺ mice depletes migrating CD103⁺CD326^– and CD103⁺CD326⁺ cDCs in skin DLN. (A) Representative dot plots of DC subsets in Zbtb46^GFP CLEC4C-DTR^– and Zbtb46^GFP DTR⁺ mice 24 h after DT treatment based on expression of GFP, CD11c, MHCII, CD326, and CD103. (B) Quantification of percentages of total cDCs (CD11c⁺GFP^high) and percentages of reduction of CD103⁺CD326⁺ and CD103⁺CD326^– DCs. Percentages of reduction were calculated because of cage-dependent variations in migrating cDC subsets. CLEC4C-DTR⁺: n = 4, DTR^– n = 5. (C) Depletion of SIGLECH⁺GFP^– and SIGLECH⁺GFP^int cells but not SIGLECH⁺GPF^high cells in Zbtb46^GFPCLEC4C-DTR⁺ versus Zbtb46^GFPCLEC4C-DTR^– mice treated with DT. One experiment representative of two is depicted.