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. 2021 Jan 11;118(3):e2023776118. doi: 10.1073/pnas.2023776118

Fig. 5.

Fig. 5.

Treatment with various proteases generates antiviral cystatin C fragments. (A) Human cystatin C protein was digested with the indicated proteases. Digestion products were separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and visualized by Coomassie Brilliant Blue staining. As controls, nondigested cystatin C as well as the synthesized CysC95-146 were included. (B) Proteases and nondigested protein were removed by ultrafiltration with different kilodalton cutoffs for purification. GHOST-GPR15 cells were then treated with the digestion products or CysC95-146 or full-length cystatin C and subsequently infected with a SIVmac239 luciferase reporter virus. Infection was measured at 3 dpi as described before. Results are displayed as means ± SD of one experiment in triplicate. #P = 0.06; *P < 0.05; **P < 0.01 (Mann–Whitney U test, unpaired t test, nonparametric). (C) Heat map visualization of identified cystatin C fragments in samples digested with the indicated proteases by mass spectrometry.