Fig. 2.
ChIP assay to determine the HBx protein-mediated recruitment of m6A methyltransferases onto HBV cccDNA and PTEN locus on the chromosome. (A and B) PHHs were infected with HBV or HBV x-null particles. Cross-linked chromatins prepared 10 d after infection were immunoprecipitated with anti-HBx or anti-METTL3 or anti-METTL14 antibodies and analyzed by qRT-PCR (A) or semiquantitative PCR (B). (C and D) Chromatins extracted from HBV- or HBV Δx-infected HepG2-NTCP cells were immunoprecipitated with anti-HBx or anti-METTL3 or anti-METTL14 antibodies. Immunoprecipitated chromatins were analyzed by qRT-PCR (C) or semiquantitative PCR (D). In all panels, data are mean ± SD (**P < 0.01; ***P < 0.001; unpaired one-tailed Student’s t test).