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Antibody Specifications for Immunofluorescence Staining
| Protein marker | Dilution | Source |
|---|---|---|
| villin | 1:100 | mouse monoclonal ab3304, Abcam |
| E-cadherin | 1:500 | mouse monoclonal 610181, BD Biosciences |
| sucrase isomaltase | 1:100 | goat polyclonal SC-27603, Santa Cruz |
| mucin 2 | 1:50 | mouse monoclonal SC-7314, Santa Cruz |
| lysozyme | 1:1000 | rabbit polyclonal A0099, Dako |
| secondary | Alexa Fluor-conjugated donkey anti-mouse/rabbit, Invitrogen | |
