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. 2020 Nov 19;10(1):305–316. doi: 10.1002/cam4.3601

FIGURE 3.

FIGURE 3

MiR‐362 regulates the proliferation and apoptosis of cervical cancer cells by targeting BAP31. (A) Apoptosis was analyzed by flow cytometry at 48 h post transfection with NC, miR‐362 mimics, miR‐362 mimics+BAP31 plasmid, and BAP31 siRNA. NC group acted as a NC and siBAP group as positive control. (B) Automated western immunoblotting analyses for caspase 3 and caspase 6 protein levels following transfection of NC, miR‐362 mimics, miR‐362 mimics+BAP31 plasmid, and BAP31 siRNA into HeLa cells. (C) The real‐time cell counting after transfection in the three groups. * showed the p value between mimics versus NC group. (D) Immunoblotting analyses for cyclin family protein levels following transfection in HeLa cells. (E) Flow cytometric analysis of the HeLa and SiHa cell cycle distributions with PI at 48 h post transfection with NC, mimics, miR‐362 mimics+BAP31 plasmid and siBAP. *p < 0.05; **p < 0.01; ***p < 0.001. Data are represented as the mean ± SD of three independent experiments