FIGURE 5.

BAP31/SPTBN1 complex regulates cervical cancer cell behavior via TGFβ/Smad pathway. (A) Differentially expressed genes were identified in the HeLa wild‐type and knockout BAP31 cell by gene microarray. (B) KEGG pathway analyses of the gene chip using the IPA commercially available software. (C) Automated western immunoblotting for Smad family protein levels following transfection of NC, mimics, miR‐362 mimics+BAP31 plasmid, and siBAP into HeLa cells. (D) Genes were ranked according to their degree of correlation with BAP31 after co‐IP and MS analysis. (E) Immunoblotting analyses for SPTBN1 and BAP31 protein levels after transfection in HeLa cells. (F) Automated western immunoblotting for SPTBN1 and BAP31 protein after co‐IP with each other in HeLa cells. (G) Immunoblotting for SPTBN1, Smad 3, and BAP31 after co‐IP. (H) Immunofluorescence staining of HeLa cells with anti‐BAP31 and anti‐SPTBN1