Figure 4.
Downregulation of NaV1.5 expression suppresses the metastatic capability of ovarian cancer cells. A, qRT‐PCR, (B) Western blot, and (C) Immunofluorescent staining were used to detect the knockdown efficiency of NaV1.5 siRNAs (−1, −2, and −3) after transfection of SKOV3 cells. D, qRT‐PCR and (E) Western blot analysis of EMT markers (E‐cadherin, N‐cadherin, and Vimentin) in scrambled siRNA and NaV1.5 siRNA‐1 transfected cells. GAPDH was used as an internal control. F, Scratch assay, (G) Transwell migration and matrigel invasion assays were performed to detect the cellular motility of SKOV3 cells. H, qRT‐PCR and (I) Gelatin zymography showed the mRNA expression level and enzymatic activity of MMP‐2 and MMP‐9 after scramble siRNA and NaV1.5 siRNA‐1 transfection. Bars represent 20 μm (C) and 100 μm (G). Data were presented as mean ±SEM (n = 3); *p < 0.05, **p < 0.01