FIGURE 3.

Periplocin attenuated the migration and invasive ability of human pancreatic cancer cells. A, CFPAC1 cells were treated with 0,125, and 250 nm periplocin for 24 h for transwell migration experiments and for 48 h for transwell invasion experiments. In the transwell invasion experiment, the cells in the medium containing 5% fetal bovine serum were seeded into the upper cavity of a Matrigel‐coated polycarbonate membrane filter (8 μm pore size). B, PANC1 cells were treated with 0,125, and 250 nm periplocin for 24 h for transwell migration experiments, and 48 h for transwell invasion experiments. Scale bar, 200 μm. The results are the mean ±SD of independent experiments performed in triplicate. p ≤ 0.05 was considered to be statistically significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the control