FIGURE 4.

Periplocin induces apoptosis of human pancreatic cancer cells. A and B, CFPAC1 and PANC1 cells were treated with periplocin at 0, 125 nm, and 250 nm for 24 h. The annexin V‐FITC assay was performed to determine the apoptotic rate of cells treated with periplocin at different concentrations. The results were analyzed by flow cytometry. C and D, The proteins expressed by the treated human pancreatic cancer cells were examined by Western blotting. Apoptosis‐associated proteins (cleaved caspase 8, cleaved caspase 3, Bax, and Bcl2) were detected using the corresponding antibodies with GAPDH as the control. The results are presented as the mean ±SD of independent experiments performed in triplicates. p ≤ 0.05 was considered to be statistically significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus the control