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. 2021 Jan 9;22(2):598. doi: 10.3390/ijms22020598

Figure 3.

Figure 3

K2 Transfection Reagent K2TR optimization for the adenoviral transduction of murine MSC. The efficacy of K2TR to increase the yield of the adenoviral transduction of MSC derived from NOD (A), C57BL/6 (B), and BALB/c (C) mice were determined as % of GFP-positive cells (green lines). The cell death was determined by PI incorporation and expressed as % of PI-positive cells (red lines). On the left side of each graph, the GFP-positive cells and the cell death for the untransduced cells (−AdV) and for the cells transduced with 250 TU/cell adenovirus alone (+AdV) were illustrated, linked by a dotted line. Adenoviral particles were complexed with various K2TR doses. MSC were incubated with 10 μL/mL K2 Multiplier (K2M) and then the complexes of adenovirus–K2TR were added to the cells. The percentage of GFP-positive cells (green lines) induced by transduction and the percentage of PI-positive cells (red lines) were determined 48 h after transduction, by flow cytometry.