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. 2021 Jan 12;13(2):257. doi: 10.3390/cancers13020257

Figure 2.

Figure 2

Exogenous activation of T cells increases pri-miR-181b and pri-miR-181a expression levels in CLL cells. (A–C) Relative gene expression values in purified CLL cells co-cultured with activated vs. non-activated T cells. Purified CLL cells were mixed with either non-activated or activated (by anti-CD2, -CD3, -CD28 antibodies) T cells (CD4+) from healthy donors (THD) at a T/B ratio of 1:1. After 4 and 24 h CLL were re-purified and assayed for gene expression. (D–F) Relative gene expression values in purified CLL cells isolated from activated or non-activated PBMCs from CLL patients. PBMCs were activated as described above and then cultured for additional 24 or 48 h. Relative expression values were determined by RT-qPCR; pri-miRNAs data were normalized to the endogenous references ACTB with the 2−Δct method. For each patient, the relative expression of pri-miRNAs was normalized to the level of non-activated sample. Data are presented as mean ± SEM and technical replicates are shown for each sample as black dot (●). p-values were calculated using Wilcoxon test in activated vs. non-activated CLL cells (* p < 0.05).