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. 2021 Jan 12;13(2):257. doi: 10.3390/cancers13020257

Figure 3.

Figure 3

MiR-181b and miR-130a are up-regulated by CD40–CD40L interactions. (A) Relative expression by RT-qPCR of miR-181b and miR-130a in pure CLL cells co-cultured in a transwell with either activated or non-activated healthy CD4+ T cells for 4 h. B cells were seeded in the upper and T cells in the lower chamber (ratio T:B, 1:1). Expression data were obtained from four different patients. (B) MiR-181b and miR-130a and (C) pri-miR-181b and pri-miR-130a relative expression by RT-qPCR in purified CLL cells from CLL patients after 4 h of co-culture with either activated or non-activated healthy CD4+ T (ratio T:B, 1:1). T cells were pre-incubated with anti-CD40L antibody or the control IgG isotype. (C) Data are presented as mean ± SEM and technical replicates are shown for each sample as black dot (●). p-values were calculated using Student’s t test (* p < 0.05, ** p < 0.01 and *** p < 0.001).