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. 2021 Jan 12;13(2):257. doi: 10.3390/cancers13020257

Figure 5.

Figure 5

MiR-181b promotes the death of leukemia cells by inducing cytotoxic lymphocytes (CTL) maturation. (A) MEC-1 cells infected with either LV AS miR181b or LV AS CTRL were co-cultured with CD40L-HeLa. After three days, stimulated and transduced MEC-1 cells were mixed with CD3+ cells from healthy donor 23 (THD23) to generate CTLs. Percentage of CD38+/CD3+ cells were measured after 8 days of co-culture. (B) MEC-1 cells infected with either LV miR-181b or LV CTRL were cultured with or without healthy activated T cells from healthy donor 22 (THD22) (E/T ratio, 1:9) in the upper transwell chamber. In the lower chamber, T cells from the same healthy donors were seeded. After eight days, percentage of CD38+/CD3+ cells were measured in the lower transwell chamber. (C) Percentage of CD8+/GrZB+ cells from healthy donors 28 and 29 (THD28 and THD29) after generation of effector CTL assay as described in B. (D) Percentage of fresh VPD+/AnnV+ MEC-1 cells co-cultured for 1 h with CTLs generated in D; E/T ratio, 2:1. Data (A–D) are reported as floating bars (min to max) with the central line marking the mean of experimental triplicates. p-values were calculated by Student’s t test (* p < 0.05, ** p < 0.01).