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. 2021 Jan 11;22(2):667. doi: 10.3390/ijms22020667

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of externally added zinc ionophore zinc pyrithione on the proliferation/viability and free zinc content of Bowes cell line and normal human melanocytes HEM during 72 h. Cells were exposed to zinc pyrithione in cultivation medium and (A–D) proliferation/viability was determined at individual time intervals with colorimetric WST-1 assay (measures the rate of metabolic conversion of tetrazolium salt WST-1 by mitochondrial succinate dehydrogenase in viable cells) and via measuring the proportion of S-phase cells using EdU-specific fluorescence. Values represent means ± SD of at least three experiments. # p < 0.05 Significantly lower than control at the same treatment interval with a one-way ANOVA test and Dunnett’s post-test for multiple comparisons. Free (labile) zinc content of treated cells (E) at 48 h was determined using fluorimetry of the zinc-specific dye Newport Green diacetate. Results represent means ± SD of five experiments.