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. 2021 Jan 11;22(2):667. doi: 10.3390/ijms22020667

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of external 0.5 μM zinc pyrithione on autophagy flux in explant human melanoma cultures with lower free zinc stores (A), average free zinc stores (B) and higher free zinc stores (C) during 72 h. Cells of melanoma cultures transduced with RFP-GFP-LC3B reporter system were exposed to zinc pyrithione and at regular time intervals their autophagy flux was measured by quantization of yellow (LC3B positive autophagosomes) and red (LC3B positive autophagolysosome) fluorescence and determination of the ratio of yellow/red puncta per cell. Data represent the mean ± S.D. of three independent experiments. * p < 0.05. Significantly higher than the beginning of treatment with the same concentration with a one-way ANOVA test and Dunnett’s post-test for multiple comparisons.