Figure 7.

Lysosomal activity and lysosomal membrane damage in explant human melanoma cultures with lower free zinc stores (M5), average free zinc stores (M10) and higher free zinc stores (M9) exposed to 0.5 μM zinc pyrithione during 72 h. Cells were exposed to external zinc pyrithione and (A) fluorescence emission of proteolyzed DQ-Green BSA reflecting lysosomal activity and (B) acridine orange diffuse green cytoplasmic fluorescence indicative of lysosomal membrane damage were determined fluorimetrically. Results represent means ± SD of at least three independent experiments. * p < 0.05 Significantly higher compared to the beginning of treatment with one-way ANOVA test and Dunnett’s post-test for multiple comparisons.