Figure 4.

W-BP100-induced membrane saturation in POPC:POPG (1:1) LUV. (A) Titration of POPC:POPG (1:1) LUV in HEPES buffer, containing 100 mmol dm⁻³ acrylamide, with increasing concentrations of W-BP100, at 25 ± 0.1 °C, as described by Melo et al. [32]. The breaking points in each curve, where a change of slope is observed, represent membrane saturation points. The fluorescence spectra of each titration are reported in the supplementary material (Figure S7). (B) Plotting of W-BP100 and POPC:POPG (1:1) concentrations at saturation points, and fitting to the membrane saturation model Equation (2). Data points and error bars represent the mean ± SD of three independent experiments.