Figure 5.

Acrylamide quenching of peptide fluorescence in aqueous solution and in the presence of LUV. Quenching of fluorescence of (A) BP100 and (B) W-BP100 in HEPES buffer and in the presence of POPC:POPG (1:1) and POPC LUV. Experimental data was fitted to the Stern–Volmer equation for collisional quenching, Equation (3). Data points and error bars are the Mean ± SD of three independent experiments. The fluorescence spectra for each condition are reported in the supplementary material (Figure S8).