Figure 7.

Peptide-induced permeabilization of anionic model membranes. (A,B) Leakage kinetics of carboxyfluorescein (CF) release from 60 µmol dm⁻³ CF-loaded POPC:POPG (1:1) LUV in the presence of increasing concentrations of (A,B) BP100 and (C,D) W-BP100, along 30 minutes of incubation in HEPES buffer, at 25 ± 0.1 °C. Asterisks and arrows mark the time-points of peptide and Triton-X addition, respectively. (E) Percentage of CF release as function of peptide concentration and P/L ratio. LC₅₀ represents the peptide concentration that promotes 50% leakage of CF. Data points and error bars represent the mean ± SD of at least two independent experiments.