Figure 5.

CHE increased TOM20, OPA1, and MFN2 protein expression and decreased DRP1 and P-DRP1 protein expression. The cells were pre-treated with 5 μM CHE or 0.1% DMSO (vehicle control) for 2 h and then incubated with or without 500 μM H₂O₂ for an additional 24 h before being subjected to Western blot analysis. (A) Compared with ARPE-19 cells treated with H₂O₂-only and pre-treated with CHE and H₂O₂, CHE increased TOM20, MFN2, and OPA1 protein expression and decreased DRP1 and P-DRP1 protein expression. Protein levels of TOM20, MFN2, OPA1, DRP1, and P-DRP1 were quantified via Western blotting. (B) Representative Western blot results. ** p < 0.01, *** p < 0.001, versus control group; # p < 0.05, ### p < 0.001, versus the H₂O₂-treated group.