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. 2021 Jan 8;22(2):580. doi: 10.3390/ijms22020580

Figure 3.

Figure 3

Effect of high concentrations of Promerim®30, 40, and 60 on the mitochondrial activity of equine articular chondrocytes cultured in the absence of fetal calf serum (FCS). Equine articular chondrocytes were amplified and seeded at P3. At 80% of confluency, the cells were treated with Promerim®30 (A), 40 (B), 60 (C) at several concentrations (10, 50, 100, and 250 μg/mL) in the absence of FCS and then cultured for 24, 48, and 72 h in hypoxia. Controls with 0%, 2% and 5% of FCS, and a death control (triton-induced death) were included. The levels of formazan were measured (OD) in the media after 24, 48 and 72 h of culture (XTT kit, Roche). Data are represented as box plots (n = 5). Statistical analyses were based on the Mann–Whitney test (* p < 0.05; ** p < 0.01) and the 0% FCS condition was used as a reference. P30, P40 and P60: Promerim®30, Promerim®40, and Promerim®60.