Figure 4.

Representative images for TCID₅₀ (Tissue Culture Infection Dose, 50%). Here, a representative image for EBOV (left), SUDV (middle), and MARV (right) is shown. Each column is a replicate (From the top), with each descending row increasing in dilution. The directional arrowhead denotes the virus concentration gradient, from high (top) to low (bottom) virus concentration. Titer is estimated by gross cell death within the replicates of each dilution using the Reed–Muench calculation [5].