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. 2021 Jan 12;9(1):156. doi: 10.3390/microorganisms9010156

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Titer comparison for the three Filoviruses in each assay. Assays were completed in EBOV (left), SUDV (middle) and MARV (right). Virus titers were determined by each assay type and compared. For NRAO and CVMO assays, titer is PFU/mL. For TCID₅₀ the assay end point was determined using the Reed–Muench method to calculate when 50% of wells would show complete cytopathology. FFU is calculated from the number of infected cell foci to give FFU/mL. Mean value with standard deviation is shown. Replicates (n) shown: MARV n = 12 (NRAO, CVMO, TCID₅₀), n = 6 (FFU). EBOV, SUDV n = 4 (NRAO, CVMO, TCID₅₀), n = 2 (FFU). Symbols (•) = NRAO assay replicate, (■) = CVMO assay replicate, (▲) = TCID50 assay replicate, (▼) = FFU assay replicate.