Skip to main content
. 2021 Jan 12;26(2):358. doi: 10.3390/molecules26020358

Figure 1.

Figure 1

Subcellular distribution of G6PDH in T. cruzi epimastigotes (strain Adriana). (A) Western blot analysis of different amounts of recombinant TcG6PDH (7, 10, 28, 50, 70 and 100 ng) and cell extracts of oxytetracycline induced (5 or 10 μg/mL: 1 × 107 cells/lane) and non-induced (0 μg/mL: 1 × 106 cells/lane) trypanosomes. Confocal microscopy of samples from induced (B–I) and non-induced (J–M) parasites. (C,G,K) Indirect immunofluorescence using anti-TcG6PDHL and secondary anti-mouse serum conjugated to Alexa 488 (green signal) or Alexa 594 (red signal), respectively. (D,H,L) Mitochondrial (K) and nuclear (N) DNA was stained with DAPI (blue signal). (F,J) signal corresponding to the mitochondrial lipoamide dehydrogenase (M) detected with specific anti-serum against the T. cruzi protein. (E,I,M) Merge images of the fluorescence staining.