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. 2021 Jan 12;26(2):358. doi: 10.3390/molecules26020358

Figure 2.

Figure 2

In situ detection of G6PDH activity in T. cruzi overexpressing G6PDH. Epimastigotes (T. cruzi strain Adriana) were grown for 24 h in the presence of 10 μg/mL oxytetracycline to induce overexpression of TcG6PDHL (Western blot shown in Figure 1A). The formazan fluorescent signal (green color) generated by the NADPH-dependent reduction of 5-cyano-2,3-ditolyl-tetrazolium chloride corresponds to G6PDH activity. (A) Bright field image with light dense intracellular particles corresponding to precipitated formazan salt. (B) Fluorescent formazan signal. (C) Cell membranes stained with CellMask. (D) Merged image of the fluorescence staining.