Figure 3.

In situ detection of G6PDH activity in T. cruzi strain CL Brenner. Epimastigotes were incubated for 24 h in the absence (no treated) or presence of 25 and 100 μM epiandrosterone (EA). G6PDH activity was measured in cell extracts using the standard enzyme assay (specific activity expressed as U/mg) and detected at intracellular level using a couple cytochemical assay based on the reduction of 5-cyano-2,3-ditolyl-tetrazolium chloride (CTC; red color). Hoechst 3342 (green color) was used to stain nuclear and mitochondrial DNA. Bright field and merged images are also shown with the white bar indicating a length of 10 μm.