Figure 2.

Terminal galactose screening for rC1-INH using RCA₁₂₀. (A) Inhibition of desialylated C1-INH to RCA₁₂₀ by pdC1-INH, rC1-INH and desialylated C1-INH as positive control. (B) Inhibition of desialylated C1-INH to RCA120 by rC1-INH, passed over an RCA120 column to remove terminal galactoses (rC1-INH RCA FT) and with rC1-INH eluted from the RCA120 column (rC1-INH RCA eluate). We can see that the capacity of inhibition by rC1-INH RCA FT is similar to pdC1-INH (dotted line), while the inhibitory capacity of rC1-INH RCA eluate is similar to desialylated C1-INH (solid line). Values are means ± SD of three independent experiments. * p < 0,05; **** p < 0,001 (according to two-tailed Students’ T-test).