Skip to main content
. 2021 Jan 11;13(2):239. doi: 10.3390/cancers13020239

Figure 3.

Figure 3

Differentiated tumor cells treated with anti-MICA/B mAb increased IFN-γ secretion of IL-2 activated NK cells. Freshly purified NK cells from healthy individuals were left untreated (A), treated with IL-2 (1000 U/mL) (B), or treated with a combination of IL-2 and anti-CD16 mAb (3 μg/mL) (C) for 18 h. OSCCs and OSCSCs were treated with anti-MICA/B mAb (5 μg/mL) for 18 h, excess unbounded antibodies were removed, and the tumor cells were co-cultured with NK cells. The supernatants were harvested from the co-cultures after 24 h, and the concentrations of IFN-γ were determined using single ELISA (n = 2) (A–C). The ratios of IFN-γ secretion of IL-2 activated NK cells induced by untreated or anti-MICA/B mAb treated oral tumor cells (OSCCs and OSCSCs) were determined as fold increase (n = 3) (D). *** (p value < 0.001), ** (p value 0.001–0.01), * (p value 0.01–0.05).