Skip to main content
. 2021 Jan 12;10(1):134. doi: 10.3390/cells10010134

Figure 4.

Figure 4

EGF signaling changes are sustained in day-90 astrocytes. The expression of selected DEGs and EGF signaling components are tested in day-60 and day-90 astrocytes. (A) PCR products of DEGs CALB1, EFEMP1, BAALC, BST2, ERRFI1 and AREG; INOS which is a downstream target of EGF signaling; and housekeeping gene EIF4G2. Most of the differences in day-60 astrocytes are still observed at day 90. (B) Bands of EGF signaling components were quantified and corrected for housekeeping gene expression. Significant changes were only observed in AREG and TGFA in day-60 astrocytes, but all genes show a trend to increased expression in day-90 astrocytes. (B) Data points represent data for each iPSC line with solid data points representing the mean per condition ± SEM. * = p < 0.05, statistical test: independent samples t-test.