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. 2021 Jan 12;10(1):134. doi: 10.3390/cells10010134

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Organoids contain a mix of neural cell populations. Immunostainings at different time points show that a mix of neural cell populations develop in organoids. The presence of neurons is shown by staining for NeuN (A), dendritic marker MAP2 and axonal marker SMI312 (B). Oligodendrocyte progenitor cells (OLIG2⁺) and oligodendrocytes (CC1⁺) are present at day 30 and day 60, while mature MBP⁺ oligodendrocytes are only observed at day 90 (C). Astrocytes were present from day 30 onwards, as shown by staining for SOX9 (A), GFAP, CD44 (D), Nestin (E) and ID3 (F). PH3 staining shows proliferating cells in day 60 and day 90 organoids (F). (A,F) Scalebar = 200 μm. (B) Scalebar = 100 μm. (C–E) Scalebar = 50 μm.