. 2021 Jan 8;22(2):578. doi: 10.3390/ijms22020578

Table 3.

Effects of the experimental treatments on the differential apoptotic cell (AC) count measures of day 8 blastocysts.

Treatment ¹	No. of Blast. ²	Cell Number			ACR (%) ⁶
Treatment ¹	No. of Blast. ²	TCN ± SE ³	ICM ± SE ⁴	TE ± SE ⁵	ACR (%) ⁶
(1) Control	42	135.97 ±1.37 ^d	36.97 ± 0.66 ^d	99.00 ± 0.95 ^d	12.49 ± 0.19 ^a
(2) Negative control	46	138.23 ± 1.31 ^d	38.71 ± 0.63 ^d	99.52 ± 0.91 ^d	11.59 ± 0.18 ^b
(3) FF EVs SEC	48	149.02 ± 1.28 ^c	41.75 ± 0.61 ^c	107.27 ± 0.89 ^c	8.06 ± 0.18 ^c
(4) AOF EVs SEC	46	153.00 ± 1.31 ^c	42.97 ± 0.63 ^bc	110.02 ± 0.91 ^c	7.85 ± 0.18 ^c
(5) FF EVs + AOF EVs SEC	47	152.48 ± 1.30 ^c	43.12 ± 0.62 ^bc	109.36 ± 0.90 ^c	6.55 ± 0.18 ^d
(6) FF EVs ODG UC	51	161.94 ± 1.24 ^b	44.39 ± 0.60 ^b	117.54 ± 0.87 ^b	6.18 ± 0.17 ^d
(7) AOF EVs ODG UC	58	166.93 ± 1.17 ^ab	46.91 ± 0.56 ^a	120.01 ± 0.81 ^ab	6.60 ± 0.16 ^d
(8) FF EVs+ AOF EVs ODG UC	56	168.69 ± 1.19 ^a	47.01 ± 0.57 ^a	121.67 ± 0.83 ^a	5.94 ± 0.16 ^d

¹ The treatments (eight groups) are defined in detail in the Experimental Design of the Materials and Methods. ² Total number of day 8 blastocysts fixed for the differential cell count. ³ Total cell number (TCN): inner cell mass (ICM) plus trophectoderm (TE) (mean ± SE). ⁴ Inner cell mass (mean ± SE). ⁵ Trophectoderm (mean ± SE). ⁶ Apoptotic cell ratio (ACR) (mean ± SE). ^{a, b, c, d} Values in the same column with different superscripts differ significantly (p < 0.05).