Figure 4.

fga exon 2 skipping. (A) A scheme to highlight changes in fga exon 2 genomic DNA in red (gDNA) led to exon 2 skipping after transcription and splicing. A partial cDNA sequence to show the exon 1 to exon 3 splicing is shown. (B) Immunoblotting of plasma samples from fga^+/+, fga^+/Δ19–56, and fga^{Δ19–56/Δ19–56} fish with anti-ceruloplasmin and anti-fibrinogen Aα chain antibodies. (C) Amino acid alignment of the predicted fibrinogen Aα chains resulting from the human FGA IVS2+3insCAT mutation and the zebrafish fga^Δ19–56 mRNA. The junction of exon 1- and exon 3-encoded amino acids are in bold and underlined for each chain.