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. 2021 Jan 10;13(1):87. doi: 10.3390/v13010087

Figure 5.

Figure 5

A111/114R-catalyzed hydrolysis of UDP-sugars. Time-course experiment with the full-length recombinant A111/114R protein (6 µg) in the optimized buffer consisting of 0.1 M MOPS-NaOH (pH 7.0), 10 mM MgCl2, and 100 µM each of UDP-Gal, UDP-Xyl, GDP-Fuc, and Glc for 16 h at 16 °C. The release of UDP was detected by the UDP-GloTM assay. Data are representative from three independent replicates, and error bars represent standard deviation.