Table 2.
Detection of vaccine-virus in feather tip pulps or spleen samples in the vaccinated group.
| Age of Chickens (Day) | 7 | 15 | 20 | 36 | 43 | ||
|---|---|---|---|---|---|---|---|
| Sample type | Feather pulp | Feather pulp | spleen | Feather pulp | spleen | spleen | Feather pulp |
| Positivity a | 90% | 100% | 100% | 100% | 100% | 100% | 58% |
| Positivity (positives/tested) | 18/20 | 20/20 | 10/10 | 20/20 | 20/20 | 12/12 | 7/12 |
| Ct value mean ± STD (rHVT-ND) | 31.0 ± 3.8 | 24.8 ± 3.8 | 29.0 ± 0.9 | 29.7 ± 5.6 | 30.6 ± 1.9 | 31.6 ± 3.3 | 36.8 ± 3.6 |
| Ct value mean ± STD (ovotransferrin) | 23.1 ± 0.7 | 23.2 ± 0.9 | 18.9 ± 0.5 | 23.5 ± 0.9 | 18.9 ± 0.9 | 18.8 ± 0.6 | 24.0 ± 1.5 |
| ΔCt b | 7.9 ± 3.6 | 1.6 ± 3.8 | 10.1 ± 1.2 | 6.2 ± 5.8 | 11.7 ± 1.9 | 12.9 ± 3.2 | 12.8 ± 3.7 |
a all samples with Ct value below 40 was considered positive, b relative quantification of rHVT-ND (ΔCt = Ct rHVT-ND − Ctovotransferrin).