Table 4.
Challenge virus shedding (measured with one-step RT-real-time PCR).
| Age at Challenge | Route of Challenge | Sub-Group | Oro-Nasal Swabs | Cloacal Swabs | ||||
|---|---|---|---|---|---|---|---|---|
| Ct (Mean ± STD and Range) |
p-Value § | Positivity # | Ct (Mean ± STD and Range) |
p-Value § | Positivity # | |||
| 20 DOA * | i.m. | V/a (vaccinated) |
35.0 ± 3.2 (25.8–36.0) |
p < 0.001 | 1/10 | 34.4 ± 3.4 (26.7–36.0) |
p < 0.001 | 2/10 |
| C/a (control) |
16.2 ± 0.9 (14.4–17.5) |
10/10 | 19.4 ± 1.6 (16.6–22.5) |
10/10 | ||||
| i.n. | V/b (vaccinated) |
29.8 ± 3.2 (26.2–34.9) |
p < 0.001 | 10/10 | 35.4 ± 1.3 (32.4–36.0) |
p < 0.001 | 2/10 | |
| C/b (control) |
19.7 ± 1.4 (18.4–22.6) |
10/10 | 22.3 ± 2.0 (19.3–26.2) |
10/10 | ||||
| 28 DOA | i.m. | V/c (vaccinated) |
35.8 ± 0.7 (33.8–36.0) |
p < 0.001 | 1/10 | 36.0 ± 0.0 (36.0–36.0) |
p < 0.001 | 0/10 |
| C/c (control) |
16.7 ± 1.6 (14.2–19.0) |
10/10 | 21.0 ± 2.6 (16.6–23.9) |
10/10 | ||||
| i.n. | V/d (vaccinated) |
30.1 ± 4.1 (25.2–36.0) |
p < 0.001 | 9/10 | 36.0 ± 0.0 (36.0–36.0) |
p < 0.001 | 0/10 | |
| C/d (control) |
18.8 ± 1.0 (16.8–20.3) |
10/10 | 23.9 ± 2.2 (19.4–27.0) |
10/10 | ||||
* DOA: days of age, # Positivity limit: Ct below 36, § Mann-Whitney test result for comparison of Ct values of vaccinated and corresponding non-vaccinated control groups.