Figure 2.

Effects of FAM188B knockdown on cell aggregation and apoptosis upon cell detachment. (A–D) Cells were transiently transfected with either si-NC or si-FAM188B for 48 h, and then cells were grown in the HEMA-coated plate for suspension culture for 24 h. Cells were then processed for immunoblot analysis using indicated antibodies (A), cell growth analysis by MTS (B), calcein- acetoxymethyl (AM)/PI staining for live/dead cell assay at indicated times (C), or annexin V/PI staining for apoptosis assay (D) as described in the “Materials and Methods”. The levels FAM188B were quantified by densitometry and normalized to GAPDH (A, right panel). Data are presented as mean values from three measurements, and error bars represent standard deviations. (* p < 0.05, ** p < 0.01 versus si-NC). Scale bars = 100 μm. These experiments were performed three times independently with similar results.