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. 2021 Jan 7;10(2):194. doi: 10.3390/jcm10020194

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Figure A1 — Gating strategy. (a) Cells were gated according to flow stability (Time), live cells (7AAD⁻), singlets (pulse geometry to remove doublets) and size. (b) The characterization of natural killer cells (NK-cells) (CD56+CD3-, orange gate), NKT-cells (CD56+CD3+), CD56brightCD16dim (green gate), and CD56dimCD16bright (purple gate), (c) The characterization of T regulatory cells as CD25+FoxP3+CD127low. (d) The expression of CD57, CD62L, NKG2D and NKp46 in CD56brightCD16dim (green histogram) and CD56dimCD16bright (purple histogram).