Table 1.
MSs characterization in terms of production yield, mean particle size and neurotrophic factor (NTF) loading and encapsulation efficiency expressed as ng of protein per mg of MSs and as percentage of the initial number of proteins for microspheres preparations, respectively. Blank MSs (MSs); MSs/VitaminE(20) (MSs-E20); MSs/VitaminE(40) (MSs-E40); GDNF/VitE(20)-loaded PLGA MSs (MSs-GE20); GDNF/BDNF/VitE(40)-loaded PLGA microspheres (MSs-GBE40).
| Formulation | Production Yield (%) | Size (µm) | Encapsulation Efficiency (EE) and Protein Loading (ng/mg) | |||
|---|---|---|---|---|---|---|
| GDNF/MSs ng/mg | BDNF/MSs ng/mg | EE GDNF (%) | EE BDNF (%) | |||
| MSs-GE20 | 83.7 | 24.7 ± 0.3 | 19.2 ± 2.9 | - | 21.0 ± 3.2 | - |
| MSs-GBE40 | 84.1 | 23.9 ± 0.2 | 27.6 ± 0.7 | 12.9 ± 0.8 | 17.8 ± 0.2 | 15.41 ± 1.0 |
| MSs | 88.4 | 27.4 ± 0.8 | - | - | - | - |
| MSs-E20 | 88.1 | 25.5 ± 0.6 | - | - | - | - |
| MSs-E40 | 83.5 | 23.9 ± 1.4 | - | - | - | - |