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The inhibitory effect (72 h) of vehicle control, 5FU (0.25 µM), HAA2020 (3 µM), or their combination on the expression of mRNA of ABCB1, ABCC1, and ABCG2 in (A) HT29, (B) HT29-5FU, (C) HCT116, and (D) HCT116-5FU cells was quantified by RT-PCR. The data represent the mean ± SD of the fold change related to vehicle control (fold change = 1 dashed line, n = 2, ×2 independent experiments). p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) were considered significant.
