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. 2021 Jan 11;26(2):334. doi: 10.3390/molecules26020334

Figure 7.

Figure 7

The expression of (A) PIK3CA, AKT, and MAPK7, and (B–D) AKT, p-AKT, ERK, and p-ERK in HT29 cells, and expression of (E) PIK3CA, AKT, and MAPK7, and (F–H): AKT, p-AKT, ERK, and p-ERK in HT29-5FU cells. All cells were treated with vehicle, 5FU (0.25 µM), HAA2020 (3 µM), and their combination for 72 h. Results in (A) and (E) represent the mean ± SD of the mRNA fold change related to vehicle control (fold change = 1 dashed line). Results in (C,D,G,H) represent the mean ± SD of the fold change percentage (%, Y axis) of the relative protein levels normalized to GAPDH, n = 2, ×2 independent experiments. p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) were considered significant.