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. 2021 Jan 11;26(2):334. doi: 10.3390/molecules26020334

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Induction of apoptosis in HT29 and HT29-5FU cells (mean ± SD, n = 3, x3 independent experiments), which were treated (72 h) with either (A,F) vehicle, (B,G) 5FU (0.25 µM), (C,H) HAA₂₀₂₀ (3 µM), or (D,I): their combination. Stacked histograms of the different drug treatments in (E): HT29 and (J): HT29-5FU cells. C1: necrosis, C2: late apoptosis, C3: live cells, C4: early apoptosis.

Induction of apoptosis in HT29 and HT29-5FU cells (mean ± SD, n = 3, x3 independent experiments), which were treated (72 h) with either (A,F) vehicle, (B,G) 5FU (0.25 µM), (C,H) HAA₂₀₂₀ (3 µM), or (D,I): their combination. Stacked histograms of the different drug treatments in (E): HT29 and (J): HT29-5FU cells. C1: necrosis, C2: late apoptosis, C3: live cells, C4: early apoptosis.